ABSTRACT
Este trabalho visou avaliar os efeitos de sulfato de vincristine sobre os testículos de ratos tratados na fase pré púbere, sobretudo quanto às alterações das células de Sertoli e das células germinativas. Foram utilizados 30 animais controles e 30 tratados com sulfato de vincristine. As aplicações da droga ocorreram aos 15 dias de vida, e a eutanásia aos 40, 64 e 127 dias de vida para possibilitar a avaliação em diferentes estágios de desenvolvimento reprodutivo. Foram realizadas medidas biométricas (pesos corpóreos e testiculares), medidas morfométricas testiculares, (eixos testiculares maiores e menores, diâmetros testiculares de túbulo e lúmen seminíferos, e altura do epitélio seminífero) e estereológicas (volumes testiculares e as densidades de volume do tecido tubular e do tecido intersticial testicular). As medidas biométricas foram feitas em todos os animais do experimento, e as avaliações morfométricas e estereológicas foram realizadas em 200 túbulos seminíferos. Os resultados demonstraram que sulfato de vincristine reduz parâmetros biométricos como peso corpóreo, peso testicular e volume testicular total. Variáveis morfométricas e estereológicas como diâmetro dos túbulos seminíferos, altura do epitélio seminífero e volume dos túbulos seminíferos também foram reduzidos. Os tipos celulares mais atingidos foram as espermatogônias, espermátides tardias e células de Sertoli.(AU)
This study evaluated the vincristine sulfate effect on rat testes treated in pre pubertal stage, especially regarding the changes of Sertoli cells and germ cells. Thirty control rats and 30 rats treated with vincristine sulfate were used. The drug application occurred at 15 days of life, and euthanasia at 40, 64 and 127 days of life to enable evaluation at different stages of reproductive development. Biometric measurements were performed (body and testicular weights), testicular morphometric measures (major and minor testicular axis and of seminiferous tubule and seminiferous lumen) and stereological (testicular volumes and volume densities of the tubular and testicular interstitial tissue). The biometric measurements were made on all rats in the experiment, and morphometric and stereological analysis was carried out in 200 seminiferous tubules. The results demonstrate that vincristine sulfate reduces biometric parameters such as body weight, testicular weight and the total testicular volume. Morphometric and stereological variables as diameter of the seminiferous tubules, height of the seminiferous epithelium and volume of the seminiferous tubules were also reduced. The most affected cell types were spermatogonia, late spermatids and Sertoli cells.(AU)
Subject(s)
Animals , Rats , Rats, Inbred Strains/abnormalities , Vincristine/adverse effects , Testis/abnormalitiesABSTRACT
La infección por Strongyloides stercoralis puede desarrollarse de manera asintomática, sobre todo en aquellos individuos portadores de una enfermedad reumática o que estén bajo tratamiento inmunosupresor.Describimos 24 pacientes con enfermedades reumáticas (lupus eritematoso sistémico, artritis reumáticas, dermatomiositis, polimiositis) que se presentaron con síntomas que imitaron un brote de la enfermedad de base (fiebre, artritis, artralgias, serositis, compromiso renal, dolor abdominal, etc.), presentando solo 6 de ellos eosinofilia periférica. También presentamos otro paciente que concurrió a la consulta con síntomas que imitaron una enfermedad del tejido conectivo (derrame pleural y peritoneal, etc.), anemia, plaquetopenia y p-ANCA (+). El parásito se halló en los exámenes de materia fecal en la mayoría de ellos y en otros en biopsia duodenal. De los 25 pacientes, 4 fallecieron.
Strongyloides stercoralis infection may develop in individuals with asymptomaticinfection, being carriers of a rheumatic disease or receivingimmunosuppressive treatment.We describe twenty-four patients with rheumatic diseases (systemic lupuserythematosus, rheumatoid arthritis, polymyositis, dermatomyositis,etc.) presenting symptoms that mimicked a flare of the rheumatic disease(fever, arthritis, arthralgia, serous and renal involvement, abdominalpain). Only six of them showed increased peripheral eosinophils.There was another patient who came with symptoms that mimicked aconnective tissue disease, such as pleural and peritoneal effusion, andlaboratory test that showed the presence of anemia, thrombocytopeniaand positive p-ANCA. Strongyloides stercoralis was found in the majorityof them and in the other by duodenal intubation and biopsy. Four out of25 patients died.